OpenPlant Blog — OpenPlant

Independent Research Fellowships

The John Innes Centre are welcoming applications from who either hold, or wish to apply for Independent Research Fellowships (such as a UKRI Future Leaders Fellowship, or a Royal Society University Research Fellowship).

Shortlisted candidates will be invited to give a seminar at the Fellows Conference, which will be held on 7 February 2022.

To apply email a two-page summary of your research plan, a copy of your CV and arrange for three letters of recommendation to be emailed to fellows@jic.ac.uk

Closing date December 3rd 2021

For more information - https://www.jic.ac.uk/vacancies/independent-research-fellowships/

sciTAG: a label design and printing app created by scientists for scientists

Proper labelling practices in the lab is one of the first things students are taught at the practical courses. As a matter of fact, labelling is an essential part of data management for scientists while working in the laboratory. And if you ever worked in a lab, you must know how much time you usually spent to label every single tube, falcon, vial, bottle etc.

The Problem

Appropriate labelling is an essential research practice. It is fundamental to day-to-day operation and long-term sample storage in laboratories. Creating labels with handwriting is convenient, adaptable, and often the standard method scientists use. However, variabilities in label format and illegible messy handwriting can both lead to flawed and/or erroneous communication, resulting in, for example, loss of important biological samples. Moreover, writing labels by hand for large scale experiments or a high number of samples is time-consuming and creates ergonomic stress. The problem presented called for a labelling method that is automated, user-friendly, and affordable.

The Project

We proposed to develop an IOS app that allows biological and medical laboratories to operate in a consistent and efficient manner, by making label design and printing more accessible.

We envisaged an app which is user-friendly, automated, and compatible with affordable label printers. To accomplish our goal, we followed a stepwise approach composed of three parts: user interface design, technical structure of the app and testing the app.

Efficient label creation frees scientists from the laborious task of writing labels by hand. Consistently and systematically designed labels ensure samples stored for long term are readable and used accurately. The app will be free, meaning that convenient label printing will be accessible to the wider scientific community.

What Has Been Achieved So Far?

Our team of five is composed of a molecular biologist, a microbiologist, a geneticist, a bioinformatician and a software developer. We started by self-teaching ourselves how to design and develop an app since most of us never worked on app development before.

Interface design decisions were made not only to be attractive to potential users, but also to be functional and simplistic as possible. One aspect of the design is visual elements. We decided on aesthetic choices such as a colour scheme throughout the app. We considered colour blindness since 4.5% of the global population experience colour-blindness. Next, we decided on the name of the app: sciTAG. We did market research to make sure this name is not a trademark. Finally, we designed an app logo depicting a double-stranded DNA alongside the name of the app.

From left: Junyan Liu (Postdoctoral Researcher, Sanger Institute), Marta Matuszewska (PhD student, Department of Veterinary Medicine, University of Cambridge) Begum Akman (Research Associate, Department of Pharmacology, University of Cambridge), Chuqiao Gong (Software developer, EMBL-EBI), Ellis Kelly (PhD student, Department of Genetics, University of Cambridge)

Then, we identified the features that are crucial for our target audience. We divided the label design into two blocks by keeping it as simple as possible with choices included to create a good label.

From left to right: home screen, label design screen, prototype label in PDF format.

Next step was to layer the technical structure: first user input information used to create a printable object such as PDF. Second, to convert this object to a storable object within the app. Finally, the app had to be compatible with commercially available label printers.

What’s Next For The sciTAG Team?

With the help of the Biomarker Challenge funding, we sourced several label printers available in the market to test sciTAG app. Among these printers only one of them is specific for lab settings (very expensive!) and others are highly affordable printers that we sourced from different companies. We distributed these printers within the team, and we are planning to complete the sciTAG prototype, start testing and reporting our experiences.

After the successful tests from the team, we are planning to recruit five laboratories within University of Cambridge to further test and optimize the sciTAG app. Eventually we are aiming to make sciTAG freely available to the science community on the App Store. Further down the line we also would like to consider making our app available for android users.

Synthetic Biology UK 2021

Synthetic Biology UK 2021 will be hosted by Synthetic Biology Research Centre Nottingham, and will focus on a number of burgeoning new areas of activity, including the engineering of microbial communities, microbial-plant interactions and applications for the sustainable manufacture of materials, the circular economy and climate mitigation strategies.

Date: November 22nd

For more information and to register visit:

https://www.eventsforce.net/biochemsoc/frontend/reg/thome.csp?pageID=53019&eventID=108&CSPCHD=0000010000005HOPapInI12EMFR969aLR0xa4Zi1NHfz6Lg5Pw

Publication: Exploring the Impact of Terminators on Transgene Expression in Chlamydomonas reinhardtii with a Synthetic Biology Approach

Publication: Exploring the Impact of Terminators on Transgene Expression in Chlamydomonas reinhardtii with a Synthetic Biology Approach

Exploring the Impact of Terminators on Transgene Expression in Chlamydomonas reinhardtii with a Synthetic Biology Approach

Katrin Geisler, Mark A Scaife, Paweł M Mordaka, Andre Holzer, Eleanor V Tomsett, Payam Mehrshahi, Gonzalo I Mendoza Ochoa, Alison G Smith.

Life (Basel) 2021 Sep 14;11(9):964

https://doi.org/10.3390/life11090964

Publication: Construction of DNA Tools for Hyperexpression in Marchantia Chloroplasts

Publication: Construction of DNA Tools for Hyperexpression in Marchantia Chloroplasts

Construction of DNA Tools for Hyperexpression in Marchantia Chloroplasts

Eftychios Frangedakis, Fernando Guzman-Chavez, Marius Rebmann, Kasey Markel, Ying Yu, Artemis Perraki, Sze Wai Tse, Yang Liu, Jenna Rever, Susanna Sauret-Gueto, Bernard Goffinet, Harald Schneider, Jim Haseloff.

ACS Synth Biol. 2021 Jul 16;10(7):1651-1666

https://doi.org/10.1021/acssynbio.0c00637

Publication: Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals

Publication: Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals

Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals

Li Y, Leveau A, Zhao Q, Feng Q, Lu H, Miao J, Xue Z, Martin AC, Wegel E, Wang J, Orme A, Rey MD, Karafiátová M, Vrána J, Steuernagel B, Joynson R, Owen C, Reed J, Louveau T, Stephenson MJ, Zhang L, Huang X, Huang T, Fan D, Zhou C, Tian Q, Li W, Lu Y, Chen J, Zhao Y, Lu Y, Zhu C, Liu Z, Polturak G, Casson R, Hill L, Moore G, Melton R, Hall N, Wulff BBH, Doležel J, Langdon T, Han B, Osbourn A.

Nat Commun. 7;12(1):2563

https://doi.org/10.1038/s41467-021-22920-8

Publication: Rapid and Modular DNA Assembly for Transformation of Marchantia Chloroplasts

Publication: Rapid and Modular DNA Assembly for Transformation of Marchantia Chloroplasts

Rapid and Modular DNA Assembly for Transformation of Marchantia Chloroplasts

Eftychios Frangedakis, Kasey Markel, Susana Sauret-Gueto, Jim Haseloff.

Methods Mol Biol. 2021;2317:343-365

https://doi.org/10.1007/978-1-0716-1472-3_21

Publication: The bundle sheath of rice is conditioned to play an active role in water transport as well as sulfur assimilation and jasmonic acid synthesis

Publication: The bundle sheath of rice is conditioned to play an active role in water transport as well as sulfur assimilation and jasmonic acid synthesis

The bundle sheath of rice is conditioned to play an active role in water transport as well as sulfur assimilation and jasmonic acid synthesis

Lei Hua, Sean R Stevenson, Ivan Reyna-Llorens, Haiyan Xiong, Stanislav Kopriva, Julian M Hibberd.

Plant J 2021 Jul;107(1):268-286

https://doi.org/10.1111/tpj.15292